NATS 1670 Lecture Notes - Lecture 41: Cloning, Dna Vaccination, Immunodeficiency
20 Apr 2017
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Document Summary
The virulent parental virus -> live attenuated vaccine. Live with an ability to replicate in host cells. The virus in the vaccine must be alive : to replicate in the vaccine"s cells. Storage how to store the virus. The attenuated virus can infect non vaccinated people. Measles, mumps & rubella (human host only) Attenuation was achieved by serial infection in rabbits led to a virus strain that was more virulent in the rabbit but was less so in dogs and humans. Infectively and viral ability to replicate are eliminated but antigenicity is not compromised. The virulent parental virus - fractionation - purified subunit vaccine. Cloning using yeast/bacterial cell - expression - vaccine. First generation extracted from the blood plasma of hepatitis patients. Most patients today have never seen a case of diphtheria, measles, or other once- common diseases now preventable by vaccines.
