BIOL2000 Lecture Notes - Lecture 16: Northern Blot, Western Blot, Riboswitch
16 Jan 2017
School
Department
Course
Professor
Document Summary
Synthesis and degradation of biomoleucesl help regulate mrna and portein levels. Degradation of protein: need for speed: more energy to do it faster. Rate of production and rate of desutrction. Common for bacteria bc energy cheap but slow. Least common for bacteria but controls levels of some ribosomal proteins. Activate or inhibiti protein by chemical modification. Rna abundance: northern or rtpcr: nucliec acid proce or detect rna. Transcript: akter, translatin: no change, post trans: no change. Protein abundance: western: measure abundnace of protein. Transcript: alter, translation: alter, post trands: depends on effect. Localization, mobility in western, interact w binding partners. ), translate: no change (absent>), post trans: alter. If after translation: wouldnt see change in rna. Translation control can resemble post trans control: both produce changes in protein levels without changing rna levels. Bacterial production of cyclin protein: rna levles through cell cycle are stable. Significant changes in western blot: cyclins are regulated post trans level.
