BIOL2000 Lecture Notes - Lecture 19: Dna Mismatch Repair, Nonsense Mutation, Frameshift Mutation
25 Apr 2016
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Gene expression: process of translating information in dna into functioning molecules within the cell. Beadle and tatum: one-gene, one-enzyme: each gene contains information for one protein of enzyme. If a gene is damaged, result is a mutant with a new phenotype >reveals function of that gene. Mutate one gene within the dna and gure out what the change in the gene does to the organism: vocab: non-functioning alleles= knock-out, null, or loss of function allele, sri and horowitz. Arginine: produced via a metabolic pathway requiring the action of three different enzymes. Grow each irradiated mutant on complete medium (all molecules needed to survive: 2. Same mutant mold can grow on medium missing a required molecule, can survive=it"s arginine: 3. Mold can"t grown on medium without arginine, misses the ability to make arginine on its own= arg mutants isolated: on final exam and have to give biochemical pathway. Central dogma of molecular biology: dna >rna >proteins.
