Class Notes (834,819)
United States (323,950)
Boston College (3,565)
Biology (478)
BIOL 3150 (23)
Cam (23)
Lecture

DNA Manipulation.docx

1 Page
30 Views
Unlock Document

Department
Biology
Course
BIOL 3150
Professor
Cam
Semester
Fall

Description
DNAManipulation (Chapter 2) • Different combinations of restriction endonucleases w/ different sequence specifity could be used to cut DNAat any position • Restricted DNAfragments containing compatible ends can be linked together with DNAligases (blunt end, 5 or 3’overhang sticky end) • DNApolymerase requires the existence of double stranded DNAtemplate w/ a free 3’OH end to initiate DNAsynthesis o Many also contain exonuclease activity for correcting (displaced in 5 to 3, incorrect in 3 to 5) • DNAcloning vector: a recombinant DNAmolecule carrying a desired DNAsegment of interest and c
More Less

Related notes for BIOL 3150

Log In


OR

Join OneClass

Access over 10 million pages of study
documents for 1.3 million courses.

Sign up

Join to view


OR

By registering, I agree to the Terms and Privacy Policies
Already have an account?
Just a few more details

So we can recommend you notes for your school.

Reset Password

Please enter below the email address you registered with and we will send you a link to reset your password.

Add your courses

Get notes from the top students in your class.


Submit