BIOL 3210 Lecture Notes - Lecture 8: Exergonic Process, Nuclear Pore, Malabsorption
Document Summary
Getting peptides into the air they are not normally gaseous. Move through the electric field at a rate proportional to their mass/charge ratio. Time of flight through the tube is measured. Proteases kind of like restriction enzymes cleave at specific spots in a polypeptide. If you take a polypeptide like insulin and cleave with a known protease it will cleave only at those spots and you can do mass spec on those fragments it"s a proteolytic. Only certain fragments will give the product of a very precise size that corresponds to fragments that could be predicted by a database. Fly the peptide through the 1st tube, in the 2nd is a high-speed collision with gas (noble, nonreactive gas) Polypeptide is broken one time and the peaks corresponding to the n terminus and the c terminus are measured. You"ll get a lot of peptide fragments, then you can get the computer to figure it all out for you.
