MCELLBI 110 Lecture Notes - Lecture 3: Arthur Kornberg, Nucleic Acid Thermodynamics, Deoxyribonucleoside
24 Feb 2021
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Lecture #3: dna replication: the central role of dna polymerases. Dna polymerases are the new enzymes that catalyze the formation of new dna strands. Initiation complex: initiator binding: where to start: origin, open the duplex: helicase loading (atp) in eukaryotes, dna melting (atp) in bacteria, replication bubble: dna melting (atp) in eukaryotes, helicase loading (atp) in bacteria. Iv. part 2: strand unwinding, protect exposed ssdna, assemble replisomes, bidirectional replication forks, ensure processivity, accuracy, and integrity of newly made dna, termination, biological synthesis of polydeoxyribonucleotides is carried out by dna polymerases. In the cell (in vivo), dna is synthesized in a template-directed manner. Initiation of dna synthesis requires a complementary oligonucleotide primer: the substrates that are joined together are deoxynucleotide 5"-triphosphates, dna is always polymerized in the 5" to 3" direction. Dna polymerase is made irreversible by the action of inorganic pyrophosphate.
