BIOL 401 Lecture Notes - Lecture 8: Ion Semiconductor Sequencing, Base Pair, Consensus Sequence
28 Feb 2017
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Next generation sequencing this example the +3 region is the start area. Remember: looking at genes they have to be pointing in 3" 5" direction right away you"re not in a reading frame. Imporant to find the right place to begin. Want to find the correct reading frame ~ look at +1, +2, and +3 on one strand, -1, -2, and -3 on the other strand. Pros of next generatoin sequencing: faster, cheaper, accesable. Assembly: assemble based on overlapping sequence (slide 6) Contain wells filled with small pieces of oligonucleotides. 100 million sequences of dna, incorporate one base sequence in it, then wash it off and add another base pair. Follow each spot through each round of reacions. Single molecule real time: synthesized in well big enough for only one dna strand. As base is incorporated, the laser in the detector will read the fluorofor. Only detection area is where the polymerase is.
