CAS BI 105 Lecture Notes - Lecture 12: Noncoding Dna, Central Dogma Of Molecular Biology, Intron
Transcription and Translation
DNA
•Universal code of bases
•Usually read in 3-letter “words” called codons (ex: GCA or CCG, ATG, TTA)
•Gene = segment of the DNA that codes for a single functional product (protein)
•All the DNA in 1 individual = genome (several genes)
•Humans have about 21,000 genes in their genome
•2 individuals of the same species have the same genes but different alleles
•Allele = version of a gene
•ex: one gene codes for hairline shape, two alleles to determine type of shape (round, pointed)
•2 individuals of different species may/may not have same genes
•Can determine evolutionary relationships based on DNA sequence
Genome
•Genes make up less than 5% of the human genome
•Coding regions (genes) = exons
•Non-coding regions = introns
•Simpler organisms tend to have less intron DNA than complicated organisms
•Humans have about 21,000 genes and about 3.3 x 109 base pairs
•Not the same as other animals
•It depends on the DNA coding
Drawing DNA
•S - A-T - S
•P P
•S - G-C - S
•P P
•*nitrogenous base only where sugars are
Prokaryote v. Eukaryote
•Prokaryote - DNA floats in the cell
•Eukaryote - DNA is housed in the nucleus
•Same steps of transcription & translation
•1. Make a copy of the region of DNA we are interested in
•^IN THE FORM OF RNA
•2. Take the RNA to the ribosome
•3. Ribosome will read it and pair the amino acids with their base pairs
•^creates a protein
•4. Protein comes off the strand and transforms into its 3D self
The Process
•The central dogma of biology
•DNA —> RNA —> Protein
•Transcription - take the DNA and make an RNA copy of it
•Translation - take the RNA and use it to make a protein
RNA
•Similar to DNA but has a different sugar molecule
•3 of the 4 nucleotides are the same
•Thymine (T) is replaced by Uracil (U)
•Types
•Messenger RNA (mRNA) - helps code for proteins
•Ribosomal RNA (rRNA) - structural component of the ribosome
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Document Summary
Drawing dna: s - a-t - s, p p, s - g-c - s, p p, *nitrogenous base only where sugars are. Prokaryote v. eukaryote: prokaryote - dna floats in the cell, eukaryote - dna is housed in the nucleus, same steps of transcription & translation, 1. Make a copy of the region of dna we are interested in: ^in the form of rna, 2. Take the rna to the ribosome: 3. Ribosome will read it and pair the amino acids with their base pairs: ^creates a protein, 4. Protein comes off the strand and transforms into its 3d self. The process: the central dogma of biology, dna > rna > protein, transcription - take the dna and make an rna copy of it, translation - take the rna and use it to make a protein. Transcription: a single-stranded copy of 1 gene of the cell"s dna is made, 1. Find the right place to start in the dna: 3.