CAS BI 203 Lecture Notes - Lecture 3: Enterobacteria Phage T4, Genetic Code, Transfer Rna
Document Summary
Strands are antiparallel to form the appropriate hydrogen bonds between base pairs. Bases are on the inside, sugar and phosphate on the outside. Transfer rna - specialized rna with complex secondary structure having the ability to detect codons within mrna. T4 bacteriophage mutant rapid lysis forms holes in the bacteria it infects. Mutation introduces a nucleotide into dna sequence, making a new amino acid. Introduced 3 nucleotides and reversed the sequence back to normal. In vitro experiments isolating specific nucleotides mixed with ribosomes, amino acids, trnas resulted in specific polypeptides. Rna polymerase can synthesize rna from rna templates. Retrovirus synthesizes dna from rna template through reverse transcription. More than 40% of human genome derived from reverse transcription. Required for replication of eukaryotic chromosomes (telomeres)
