CAS BI 525 Lecture Notes - Lecture 4: Ubiquitin, Cd63, Western Blot
1 Oct 2016
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Polyubiquitin molecules as tag to target protein a. ii. Activation of ubiquitin phosphorylation by e1 ligases specific biochemical modifications a. iii. E2 ligases to transfer activated ubiquitin directly to the target protein or to e3 ligases a. iv. E2 or e3 transfer activated ubiquitin directly to target protein a. iv. 1. How do they decide if e3 is necessary? a. v. When we want to look at ubiquitin status of a protein through sds page- leads to increased weight of the target molecule each shift is equal to the ubiquitin (western blot) a. v. 1. How many does a protein need is it dependent on the size of the protein: what happens to the ubiquitinated substrate after it has been ubiquitinated ? b. i. Proteasome is a highly organized system of proteins proteases b. i. 1. b. i. 2. b. i. 3. b. i. 4. Organized in to core of alpha and beta subunits. Bsa lumen of organelle: because it has a membrane, it can fuse with other membranes constant shuffling of membranes in the cell.
