CAS NE 525 Lecture Notes - Lecture 9: Cytochrome C, Lysosome, Mitochondrial Fission
10 Aug 2018
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Htt sequesters rest in cytosol, less seen in nucleus. Study: how mutant and wt htt will interfere with rest, levels of rest at cytosolic and nucleus level. Understand connection between rest relocalization and rest interaction with. Cells overexpression of htt: through transgenic mice or cell transfection. System contains both endogenous gene and inserted gene. So has endogenous htt and overexpression of htt (2 proteins). Because 50% ko mice are still viable, the endogenous htt could be enough for functioning despite the overexpression of inserted gene. Heterozygous patients can make it through development: overexpression of htt: much less rest in cytoplasm much. Htt knock in cells: shut down expression of endogenous protein and express only the inserted gene (1 protein): overexpression of htt: much less rest in cytoplasm much. More in nucelus: wt htt: rest remains in cytosol. Rest interacts with wt htt in non-hd cells, but in hd, rest interacts with htt to a lesser extent.
