BIOS 20187 Lecture Notes - Lecture 20: Sanger Sequencing, Fluorescent Tag, Chromatography
26 Dec 2019
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Lecture 1: gene sequencing technologies: sanger sequencing, uses ddntps in conjunction with dntps to create dna strands of different fragments. The ddntps are have a fluorescent tag, which is unique for each specific ddntp (a,t, g, c). When fed through the capillary (similar to gel electrophoresis), the strands of different lengths will filter out, and read through the capillary in order of size. The will help us determine the order to nucleotides: ddntps = dedioxyribonucleotides. Have no oh groups, which prevents the binding of more nucleotides, creating fragments: applications = think ptc/pcr lab from genetics final (chromatogram, shotgun sequencing, multiple copies of dna are chopped up randomly. The smaller peices are able to be sequenced. Any similar areas on the newly sequenced fragments are matched which helps solve the entire dna strand of interest (contigs). Overlapping contigs come together to help create genome.
