BIOL 153 Lecture Notes - Lecture 5: Tae Buffer, Sodium Hydroxide, Glycerol
6 Jun 2020
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Pipettes, pipetting, and dilutions: 0. 01, 1x10^-6, 1000, 100, 0. 01, 1x10^-5, you need 40ml of glycerol in 200ml of solution to get a 20% v/v because. 40ml/200ml *100 = 20%: m= moles of sub/l of solution. 2= g naoh use 2 grams of naoh in 200 ml of water: concentration of the 1x solution is 0. 01m. You will need to count how much tae buffer you need. The buffer volume needed would be: buffer1 * volume1 = buffer2 * volume2. 1* 125ml= 20 * volume2 voleu2= 125ml/20= 6. 25ml. Add the 6. 25ml buffer, then add the agarose. Since the agarose is powder, you need to weigh the amount of agarose needed. The calculation would be: weight = volume * density * percentage weight = 125 ml * 1g/ml * 0. 8%= 1g.
