3. Write the calculations to make the following solutions using the stocks and solids listed above. Assume all the above solutions are in deionized water.
a. How would you prepare a 500 mL solution containing 1.5 M Tris and 20% KCl?
b. How would you prepare a 0.75 L solution containing 50 mM NaCl and 10% SDS?
c. How would you prepare a 100 mL solution containing 1% agarose and 1x TBE?
d. How would you prepare a 250 mL solution containing 250 mM Tris, 5 g/L NaCl, and 50% SDS?
E. How would you prepare a 200 μL solution containing 1% glycerol?
A. You are given 50 µL of a highly concentrated DNA solution. You need to prepare 1.0 mL of a 1:500 dilutions. Describe how you would prepare this solution
B. You diluted a DNA solution 1:100 and measured the OD at 260 nm. The spectrophotometer gave you a reading of 0.35. Describe how would you determine the DNA concentration of your original solution? Remember, the εDNA = 50x10-3 mg/mL.
3. Write the calculations to make the following solutions using the stocks and solids listed above. Assume all the above solutions are in deionized water.
a. How would you prepare a 500 mL solution containing 1.5 M Tris and 20% KCl?
b. How would you prepare a 0.75 L solution containing 50 mM NaCl and 10% SDS?
c. How would you prepare a 100 mL solution containing 1% agarose and 1x TBE?
d. How would you prepare a 250 mL solution containing 250 mM Tris, 5 g/L NaCl, and 50% SDS?
E. How would you prepare a 200 μL solution containing 1% glycerol?
A. You are given 50 µL of a highly concentrated DNA solution. You need to prepare 1.0 mL of a 1:500 dilutions. Describe how you would prepare this solution
B. You diluted a DNA solution 1:100 and measured the OD at 260 nm. The spectrophotometer gave you a reading of 0.35. Describe how would you determine the DNA concentration of your original solution? Remember, the εDNA = 50x10-3 mg/mL.
