BIOL-1100 Lecture Notes - Lecture 15: Enzyme Catalysis, Solvation Shell, Catalysis

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An enzyme differs from a chemical catalysis: they are stereospecific, work under mild conditions- temperature, ph, aqueous soltuions. Some enzymes require extra components that include cofactors, coenzymes or prosthetic groups (holoenzyme) while some are active alone (apoenzyme). The enzyme must be specific in order to regulate a reaction because the noncovalent interactions between the active site and substrate must match up. Enzymes provide energy that allows the reaction to proceed through the free energy they release once the s binds. Stickase experiment: found that the enzyme must be an induced fit for the transition state not the ground state or else the activation energy would be increased. Enzymes provide binding energy in 4 ways: reduction of entropy (disorder), stabilization with a solvation shell, distortion of substrates, and proper alignment. Know the mechanisms of acid-base catalysis, covalent catalysis, and metal ion catalysis. Thermodynamics= heat and energy associated with the work and energy in a reaction.

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