BIOL-4670 Lecture Notes - Lecture 5: Mycosis, Lymphadenopathy, Rouleaux
Document Summary
Acute leukemias: leukemia mutation of stem cells that cause impaired blood cell production, neoplastic cells crowding marrow cause decrease in plts and rbcs, spleen extramedullay hematopoiesis. Initial evaluation: symptoms, cbc, cell lineage, cell maturity, acute immature, chronic more mature, usually in adults, categorized by cell lineage and maturity, acute myeloid leukemia, acute lymphoblastic leukemia, chronic myelocytic leukemia, chronic lymphocytic leukemia. Identify lipids/enzyme within blast population: promyelo, myleo, metamyelo, band, and seg neutros stain strongly positive, negative reaction differentiates between all from. Sudan black b: stains phospholipids and intracellular lipids, negative for lymphs, differentiates all from aml, sensitive for granulocyte precursors. Specific esterase (naphthol as-d chloroacetate esterase: primary granules of myeloid, myeloblasts, neutros, basos, and mast cells stain positive, eosino, mono, and lymphs stain negative. Nonspecific esterase (alpha-naphthyl butyrate or alpha- naphthyl acetate esterase: monocytes stain negative, used to identify monoblasts and monos in all and aml.
