BIOMG 1350 Lecture Notes - Lecture 1: Cell Theory, Transmission Electron Microscopy, Immunofluorescence

• School: Cornell University
• Department: Molecular Biology and Genetics
• Course: BIOMG 1350
• Professor: Discussion

Document Summary

1655: hooke, inventor of microscope, coins the term cell . Was actually looking at cell walls, cells were dead and empty. Antonie van leeuwenhook: built of 200 microscopes. first to observe living cells. 1838: cell theory developed by schleiden and schwann. Cells are universal building blocks of all living tissues. : all living organisms are composed of one or more cells, the cell is the most basic unit of life, all cells arise only from pre-existing cells (developed by pasteur) Resolving power: ability to distinguish two close objects. Maximum resolving power of microscope depends on wavelength of illumination. Visible light has wavelengths of 400-700 nm and can resolve objects ~200nm apart. Electron microscopes can resolve atoms because the wavelength of electrons are much shorter. Settings such as phased and polarized light can improve quality. Red dyes attach easily to negative molecules in cells. Fix sample somehow (attach it to something so it doesnt move)