BIOMG 1350 Lecture Notes - Lecture 4: Phosphatase, Guanine, Methylation
Document Summary
Proteins function by making specific interactions with other molecules: ligand-protein relationship. Fits perfectly into complementary protein shape: protein"s physical interaction with other molecules determines its biological properties, binding is specific, specific interactions depend on a number of noncovalent interactions/bonds. Protein interaction with a specific ligand: unfolded protein amino acid side chains spread out, folded protein brings side chains together to form binding site. Amino acids interact with ligands with covalent bonding. Enzymes: catalyze covalent bond formation or breakage, active site. Pocket generated that has amino acid side chains for ligand interaction. Enzyme substrate complex [catalysis] enzyme-product complex: catalysis increase rates of chemical reactions, not changed by reaction. Enzymes catalyze reactions by lowering activation energy barrier: downhill reaction , although the reaction may be energetically favorable, there is an energy barrier that needs to be overcome activation energy, enzymes lower activation energy for catalyzed reaction.