BIO 303 Lecture Notes - Lecture 25: Alternative Splicing, Rna Editing, Spliceosome

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24 Apr 2019
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For rna produced in mitochondria and chloroplasts. Excision of introns and rejoining of exons in trnas of bacteria. Intron removed and terminal ends of adjacent exons joined by ligase. In eukaroyotes, transcript of rrna intron is the source of enzymatic activity necessary for removal. Introns spliced out two exon regions ligated leads to mature rna. Self-excision of group i introns occurs in many protozoans. In eukaryotes, self-excision occurs in removal of introns from primary mrna and trna transcripts produced in mitochondria and chloroplasts. Self-excision of group i introns occurs in bacteria, lower eukaryotes, and higher plants. Pre-mrna introns are spliced out by spliceosome. Reaction involves formation of lariat structure. (figure 13-14) Snrnps: important component of spliceosomes involved in excision. Produces related protein from single gene, increasing number of products derived from single genome. Nucleotides are added/deleted from total number of bases. Identities of individual nucleotide bases are altered; prevalent in mitochondria and chloroplast.

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