BOT 4734C Lecture Notes - Lecture 4: Ribosomal Rna, Antibody, Transfer Rna
Document Summary
Transformation construct/vector: the mechanism that carries target genes to be introduced into the cell. A transformation vector consists of: gene of interest, selectable marker gene. Every gene must have a promoter, coding region, and a terminator. A construct contains selection marker gene and a gene of interest. Depends based on if they"re used for basic research or applied research (our class would be considered basic research for learning purposes, but applied research can use any number of potential genes of interest) Mode/effect of transgenes: overexpression, suppression, mutation. Regulatory or pathway genes: transcription factors, master genes, a gene of unique function. Genes for applied research: increase production. Photosynthesis, biomass, yield: reduce loss of individuals due to stress. Stress resistance to: disease, water, temperature, toxic pollution: value-added new traits: Nutrition (vitamins, proteins, amino acids, fatty acids, minerals ) Nucleic acid-based biotechnology: what are nucleic acids, dna, rna, dna, a,t,g,c, their combination/ order (sequence) on a dna strand can carry information.
