MCB 3020 Lecture Notes - Lecture 19: Cytometry, Microorganism, Antibody
Document Summary
Chapter 7: microbial growth: microbial suspension forced through small orifice with a laser light beam, movement of microbe through orifice impacts electric current that flows through orifice. Instances of disruption of current are counted: specific antibodies can be used to determine size and internal complexity. Viable counting: alive or dead: whether or (cid:374)ot a (cid:272)ell is ali(cid:448)e or dead is(cid:374)(cid:859)t al(cid:449)ays (cid:272)lear (cid:272)ut i(cid:374) (cid:373)i(cid:272)ro(cid:271)iology. Cells can exist in a variety of states (cid:271)et(cid:449)ee(cid:374) (cid:858)fully (cid:448)ia(cid:271)le(cid:859) a(cid:374)d (cid:858)a(cid:272)tually dead(cid:859) Viable counting methods: spread and pour plate techniques. Diluted sample of bacteria is spread over solid agar surface or mixed with agar and poured into petri plate. After incubation the numbers of organisms are determined by counting the number of colonies multiplied by the dilution factor results expressed as colony forming units (cfu: membrane filter technique. Bacteria from aquatic samples are trapped on membranes. Colony count determines # of bacteria in sample.
