PCB 3063 Lecture Notes - Lecture 9: Dna-Binding Domain, Tata Box, Gene Silencing
Focused Promoter
initiator is in a very defined region usually -2 to +4
TATA box
usually -30 to -24
is flanked by two transcription factors
Enhancers
*-Cis-acting
-Located on either side of a gene
-Can be inverted without significant effect
-Not specific to any gene*
Silencers
Cis-acting elements that repress the level of transcription initiation
Part of regulation is
the interaction of cis and trans elements
Transcription factors
DNA- binding domain: Must have a domain that allows it to recognize and bind to DNA sequences in the
cis-acting regulatory site
Trans-activating domain:
How do cis and trans factors act to influence transcription initiation?
...awaiting answer.
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Document Summary
Focused promoter initiator is in a very defined region usually -2 to +4. Tata box usually -30 to -24 is flanked by two transcription factors. Cis-acting elements that repress the level of transcription initiation. Part of regulation is the interaction of cis and trans elements. Dna- binding domain: must have a domain that allows it to recognize and bind to dna sequences in the cis-acting regulatory site. The bent over shape of the enhanceosome complex serves what purposes? acts as a recruiter acts a. Structural:gal 1, gal 10, gal 12, gal 7. Is always sitting on the genes regardless of activation/inactivation. If you shorten the poly a tail too much ~decreases stability nonsense nmd surveillance system that looks for these and cuts out a mutation that put a stop codon before the actual end of the mrna msg. Can generate different forms of mrna from identical pre-mrna, giving rise to a number of proteins from one gene.
