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Lecture 13

Lecture 13 – DNA Synthesis.docx

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Department
Life Sciences
Course
Life Sciences 1a
Professor
Robert Lue
Semester
Fall

Description
Oct 15 – DNA Synthesis ­ Meselson – Stahl  ▯semiconservative or conservative mechanism ­ Hybrid DNA in one round as opposed to light and heavy  ­ Triphosphate in DNA labeled alpha, beta, gamma ­ Base + sugar = nucleoside ­ Nucleoside with phosphate = nucleotide  ­ Nucleotides added onto 3’ end, chain growth in 5’ to 3’ direction ­ Base chosen by complementarity with nucleotide on opposite template strand  ­ Attack of 3’ oxygen on dCTP (cytosine triphosphate) alpha phosphate  ­ Pyrophosphate is leaving group for phosphodiester bond                    ­ 3’ important to attack alpha phosphate of next nucleotide  ­ pyrophosphate  ▯two single inorganic phosphates  ­ this reaction coupled to the sy
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