ANSC 1011 Lecture Notes - Lecture 15: Genome Editing, Recombinant Dna, Transgene
Document Summary
The transgene: from a different source, two segments: Dna sequence that codes for a specific product. A protein: hormone, enzyme, growth factor, structural protein etc. Promoter and/or enhancers: expression in many tissues, tissue specific expression, environmental signals. Production of dna: transgene inserted into plasmid, vector to insert dna into bacteria, grown in bacteria, plasmid dna isolated, purified for injection/transfection. Identification of transgenic animals: dna analysis, low percentage are transgenic, not all express transgene, random position effects, select founder animals, establish line from one or two animals, breeding, embryo transfer, cloning. Transgenic animals in medicine: production of drugs cattle, goats, chickens, transplantation of tissue into humans, unmet need for drug production, blood clotting factors, human serum albumin, human antibodies, pigs, neuronal cells, organs, disease models (test treatments on animals) With an expected global market of . 8 billion by 2016, synthetic biology will play an important role in the bio economy and has increasing implications for future us competitiveness and employment.
