MB 351 Lecture 35: 10.3 Gene Expression
Document Summary
Z,y,a: encode gene product, dna binding proteins, regulatory proteins, bind to specific sites on dna, turns transcription on or off, sequence specific interactions. Substrate binds here: has regulatory ( allosteric/effector) site, effector mol binds here, changes shape when something binds to either site, repressor proteins. Small mols bind to regulatory protein and affect activity: types. Inducers: bind to repressor and turn transcription on, co-repressors also bind to repressor bout they turn transcription off, activator, bind to activator proteins, turn transcription on, types of transcriptional regulation, negative control. Stops transcription through repressor protein which are controlled by effector molecules: repression, regulatory mechanism inhibits gene expression so enzyme not made, response to too much stuff, affects anabolic enzymes. Luxi protein made acyl homoserine lactose autoinducer (ai: the autoinducers is secreted out and at a certain concentration enter back into cell and bind with luxr. Luxr-ai complex activates luciferase operon to make bioluminescence.
