BIOL 2299 Lecture 1: Exam 1 Study Guide

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Know where stem cells are found in the developing embryo and in which stages of embryonic development stem cells are totipotent, pluripotenet and multipotent. Not found in adults not found in adults heart, spinal cord, bone marrow, and skin. Niche: locations that support stem cells, signal stem cells to remain undifferentiated, divide, or produce differentiated cells. They function to replenish body cells throughout an adult"s life by replacing deceased or aging cells. Digestive and respiratory tract, teeth, heart, blood, skin. Culture on feeder layer of mouse embryonic fibroblasts. In vivo and in vitro screening and the formation of teratomas. Molecular markers for the 3 germ layer types. Endoderm: sox 17, mix, variable depending on species. Look for oct 4 using rt-pcr (protein involved in the self-renewal of es cells) Initially active as a maternal factor in oocytes through implantation. Mouse embryos with low oct-4 will not form icm.

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