BIOL 2301 Lecture Notes - Lecture 30: Oncogene, Vascular Endothelial Growth Factor, Wild Type
9 Apr 2018
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Grow cells at permissive temperature and then shift them to non permissive temperature and essentially misfold all the proteins allowing us to see the effect of the proteins. These cells get arrested at the same point in the cell cycle. This tells us which stage these proteins are needed in. The cell cycle is regulated by cyclins and cyclin-dependent protein kinases (cdk) Expressed at different points during the cell cycle and allow the cell to progress through the cell cycle. Binding of the cyclin activates the cdk. The complex has affinity for a variety of target proteins. Rb (retinoblastoma): target of cyclin and cdk. Rb is a protein required to maintain (stop) the cell at a point in g1 (called. Start ) until the cell is large enough to go through s phase and growth promoting signals are sensed by the cell.
