BIOL 2301 Lecture Notes - Lecture 30: Smpte Color Bars, Wild Type, P53
15 Sep 2016
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When we fail to control cell cycle, that"s what causes it. Machinery to make sure everything goes right. Want two copies, don"t mess up segregation, etc. Don"t divide in middle- grow a bud. Using temperature sensitive mutants in yeast to find cell cycle genes. Find mutants that at high temperature cant get at cell cycle. Grow at low, shift temperature, keep growing until the point when they need the protein, then they stop and are all synchronized at that point in growth. All stopped at one point of cell cycle- g2-m phase in this image. The cell cycle is regulated by cyclins and cyclin-dependent protein kinases (cdk) Color bars show where they are expressed. Cyclin- help the cell progress around the cell cycle: come at specific points. Don"t know which or where they are. All cyclin-cdk complexes work in the same way. One of the critical targets of the g1 cyclin/cdk is rb (retinoblastoma protein)
