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BIOL 2301 Lecture Notes - Lecture 2: Transposable Element, Asteroid Family, Starch

65 views4 pages
indigooyster587
15 Aug 2018
School
Northeastern University
Department
Biology
Course
BIOL 2301
Professor
Christopher Richardson

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Document Summary

Gregor mendel i. gave first transmission of dear description of rules hereditary diseases the governing pre . Hypothesis: each parent passes down distinct elements of. "i principles of segregation a genes separate (segregate randomly) into gametes. Each gamete is equally likely to the pair contain each member at: gametes reunite at random in fertilization. ) individual and a recessive genes regularities i. results i. ii. iii. the of individual still used. To traits two cross: factors recession ames known) individual for the purpose oh, factors phenotypes of the at gametes statistical techniques l. Dihybrid : the different gametes determining the proportion at a looked for statistical bk the recessive in equal frequency of all an # individual hereditary . known varieties are observed in the. Advantages hybrid for in outcomes of his crosses in a dihybrid are 9 :3 :3 :1 numerical ratios of traits.

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Related Questions

When dealing with Hardy Weinberg Equations, why does the alliedfrequency always add up to 1 (p+q=1)?

- A. B. C. D. E. F. G. H. I. J. K. L. M. N. O. P.

Step 1.
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Step 2.
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Step 3.
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Step 4.
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Step 5.
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Step 6.
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Step 7.
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Step 8.
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Step 9.
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Step 10.
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Step 16.
A.

Don appropriate personal protective equipment (PPE).
B.

Aseptically transfer 1 mL from dilution tube 2 to dilution tube 3 and mix well. This is a 10-5 cumulative dilution.
C.

Aseptically transfer 1.0 mL from dilution tube 4 to dilution tube 5 and mix well. This is a 10-7 cumulative dilution.
D.

Aseptically transfer 0.1 mL from dilution tube 1 to dilution tube 2 and mix well. The cumulative dilution of the culture is 10-4.
E.

Allow the inocula to soak into the plates before proceeding.
F.

Aseptically transfer 0.1 mL from dilution tube 2 to plate A1. Using the spread plate technique, disperse the sample evenly over the entire surface of the agar.
G.

Aseptically transfer 1 mL from dilution tube 3 to dilution tube 4 and mix well. This is a 10-6 cumulative dilution.
H.

Obtain eight plates, organize them int four pairs and label them A1, A2, B1, B2, etc.
I.

Keep accurate records in your lab notebook.

Clean up the lab benches, doff PPE, and wash hands.
J.

Repeate the procedure with plate A2 and label both plates "10-5mL of original sample," or simply "10-5"

Notice that transferring 0.1 mL counts as a dilution!! The tube was a 10-4 dilution, but the plate is a 10-5 dilution!!
K.

Invert the plates so they are "agar up" to prevent condensation from ruining the plates.
L.

Repeat the aseptic transfer, spread plate technique, and label schematic for dilutions tubes 3, 4, and 5, and plates B, C, and D.
M.

Incubate the plates at 35℃ for 24 to 48 hours.
N.

Obtain five dilution tubes and label them 1–5, respectively. Make sure they remain covered until needed.
O.

Aseptically add 9.9 mL sterile water to dilution tubes 1 & 2. Cover when finished. Aseptically add 9.0 mL sterile water to dilution tubes 3, 4, and 5. Cover when finished.
P.

Mix the broth culture an aseptically transfer 0.1 mL to dilution tube 1. Mix dilution tube 1 well. This is a 10-2 dilution

In making a tissue culture medium, you are required to use final concentrations of 10μM indole-3-butyric acid(IBA) and 3μM 6-benzylaminopurine(BAP) in 1L of final medium. The formula weight of IBA is 203.2 and the formula weight of BAP(as hydrochloride) is 261.7. Write out a protocol to explain exactly how you would achieve this, including what masses(weights) of solids you would add to what volumes of which liquids and any stock solutions and dilutions you would have to make. At what stage in your medium preperation would you add the growth regulators?