BIOL-UA 21 Lecture Notes - Lecture 25: Rna-Seq, Ribonuclease H, Polyadenylation
22 Dec 2016
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Lecture 25: gene expression on the genome scale: differences in the transcriptome of nerve and muscle cells will help us understand how cells became and developed their functions as nerve and muscle cells. Differences in cell types or between two states in cells (normal v. disease) is brought about by variations in gene expression: analysis of gene expression: In-situ hybridization and northern blot have low throughput; one or few transcripts analyzed at a time. There are many more mrnas in a transcriptome; >30000. Northern blot will let us know levels of expression of a particular gene in a given type of cell; radioactively labelled probe complementary to mrna targeted is hybridized on gel. In-situ hybridization gives spacial information; how a mrna is localized in an organism: analyzing the transcriptome: Dna microarray is a collection of microscopic dna spots attached to a solid surface. Dna with speci c sequences corresponding to a gene.
