BIOL-UA 21 Lecture Notes - Lecture 3: Transcription Bubble, Phosphodiester Bond, Peptide
18 Mar 2016
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The central dogma ii: rna synthesis and properties. **rna is transcribed from specific starting points (form of self regulation) Dna and adding rntps to growing rna (3) termination (a) at transcription stop site, polymerase releases completed rna and dissociates from dna. Most important decision: when and where to start transcription promoter: place on the dna molecule where the rna polymerase binds to start initiation start site: place that is complementary to the first nucleotide of rna that is made. Bubble = region of separated strands (open complex) - polymerase can synthesize first phosphodiester bonds, linkage is made between those two. As polymerase ii advances down 3" template strand, melts duplex dna and adding rntps to grow rna. As rna grows longer and longer strand stretches out of rna polymerase. The bubble moves along with the polymerase. Bubble maintains a constant size: as it open in the front, it closes in the back.
