BIOS 1700 Lecture 15: BIOS 1700 Notes #14 PDF

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A gradient of protons creates potential energy that in turn runs atp production. F0 subunit: forms a channel that rotates as protons pass through it. F1 subunit: uses the rotational (kinetic) energy of f0 to catalyze the synthesis of atp. How was it tested that atp synthase uses the proton gradient: hypothesis: a gradient of h+ (potential energy) is used to produce atp. Experiment: materials needed: proton pump activated by a light bulb, atp synthase, liposome (to hold all the protons), h+ (protons) Adp, pi: light activates the proton pump which. As h+ leaves through atp synthase, adp + pi are turned into atp: results: a gradient of h+ is used to produce atp. When the light is off, no protons move. !1 into the cell and the protons inside the cell move out of the cell to equalize concentrations on both sides. Wednesday, september 28, 2016: this is why the graph decreases.

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