Biochemistry 2280A Study Guide - Pentose, Ketone, Statin

• School: Western University
• Department: Biochemistry
• Course: Biochemistry 2280A
• Professor: Chris Brandl

Document Summary

Proton concentration is about 10 folds higher in intermembrane than matrix (higher ph matrix) Excess positive charge builds up in intermembrane. Protonmotive force: combination of proton concentration and electrical potential cause protons to flow back into matrix if given opportunity. Chemiosmotic hypothesis: protonmotive force drives synthesis of atp. Evidence: intact membranes required for atp synthesis ad if make membrane permeable to protons, atp synthesis stops because destruction of proton gradient. Protein complex that allows protons to flow down electrochemical gradient. Energy from proton movement coupled to make atp in matrix there is a spatial separation from where protons move to where. Rotational mechanisms protons moving cause central piece to turn, which eventually makes atp coupling factor 1 f1 is attached to transmembrane segment f0 that allows protons to move across membrane. Human enzyme is very complex with many distinct subunits. F0 3 subunits ab2c10 and proton channel (allows p+ to move across membrane)