BME 20100 Lecture Notes - Lecture 9: Redox, Isozyme, Enzyme Kinetics
27 Nov 2017
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Isozyme: an enzyme with the identical function as another, but different structures. Apoenzyme: inactive enzyme, activation occurs upon binding of an organic or inorganic. Holoenzyme: active compound formed by combination of apoenzyme, prosthetic group, Ribozyme: rna enzyme that catalyzes a chemical reaction. Coenzyme: non-protein part of an enzyme, vitamin derivative cofactor, protein portion cofactor. Cofactor: metal ions or coenzymes, assist enzymes during catalysis. Prosthetic group: metal ion or organic compound covalently bound to protein. Rate enhancement = rate with enzyme / rate without enzyme. Km = -1 / (intersection at x-axis) or [s] at vmax/2. Vmax = 1 / (intersection at y-axis) V0 = (vmax * [s]) / (km + [s]) Random: random order or reactants binding and products leaving (intersect on somewhere other than y-axis) y-axis) Can be overcome by increasing substrate concentration. Noncompetitive: can bind to enzyme alone or enzyme-substrate complex. Sanger method requires: template dna, primer, dna polymerase, nucleotides (dntps),
