01:119:115 Lecture Notes - Lecture 16: Lac Operon, Trp Operon, Lactose Permease
2 May 2016
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Document Summary
Function as huge enzyme complex: binds to mrna template and forms peptide bonds between aas. 2 ribosomal subunits (large + small)are made of proteins (structure) + ribosomal rna (rrna) (catalytic function) large subunit has grooves (depressions) where trna attach a binding site: holds trna that carrier the next aa to be added. E binding site: exit site where discharged trnas leave. Translation: a. initiation mrna, trna with 1st aa, and 2 ribosomal subunits assemble 1st, small ribosomal subunit binds w/ mrna & special initiator trna (carries met) small subunit moves along mrna until reaches start codon (aug) Initiation factors (factors) bring in large subunit forms translation initiation complex. Translation: b. elongation: series of repeated cycles adds a 1 aa to growing peptide, peptidyl transderase. Ribozyme- catalyst is an rrna, not a protein. Catalyzes peptide bond between aa in p and a sites. Covalent bonds btw. a. a. and trna at p sites is broken.
