01:447:380 Lecture Notes - Lecture 3: Electroporation, Cytokinesis, Insulin-Like Growth Factor 2 Receptor
Document Summary
~f factors (plasmids) origin of transfer, origin of replication. ~heat shock to open up bacteria, electroporation, coat dna with lipids so that it dissolves with membrane. ~mendel looked at very simple characters, single genes affected each phenotype, but most phenotypes are determined by interaction of many genes. ~complex trait result of multiple genetic interactions and environment. ~recessive epistasis recessive c stops any dominant p, 9:7 ratio instead of 9:3:3:1. ~d allele dominant to k allele having any d makes yellow. ~dup dom only having all recessive alleles causes no color. ~oi can have allele without having symptoms, phenotype does not penetrate everybody. ~cytoplasm comes from mother, which is why mitochondrial dna comes from mother. ~mitochondria undergo fusion and fission based on energy needs. ~no specific, equal separation of mitochondria in cytokinesis.