BIOL 203 Lecture Notes - Lecture 18: Restriction Fragment Length Polymorphism, Polymerase Chain Reaction, Single-Nucleotide Polymorphism
Document Summary
Dna cloning preview: sequencing of the genomes in 2010. Nucleotide sequences from two different sources combined in vitro into the same dna molecule. Central to genetic engineering direct manipulation of genes for practical purposes: dna technology. The manipulation of organisms or their genetic components to make useful products. Revolutionized biotechnology: microarray: a measurement of gene expression of thousands of different genes, dna cloning: preparing well-de ned segments of dna into identical copies. Methodology: use of bacteria and their plasmids, small circular dna, replicate separately from bacterial chromosome, useful for making copies of a particular gene and producing protein product. Thursday, december 3, 2015: the dna sequence can be read from the resulting spectrogram, technique has become faster and longer strands are able to be used. Gene cloning: involved using bacteria to make multiple copies of a gene, foreign dna is inserted into a plasmid.
