BIOL 2100 Lecture Notes - Lecture 20: Cloning Vector, Dna Ligase, Recombinant Dna
9 May 2016
School
Department
Course
Professor
Document Summary
Sequencing of human genome in 2003 marked turning point in biology because it sparked remarkable technological advances in dna sequencing. Dna technology- main techniques for sequencing and manipulating dna. Biotechnology- the manipulation of organisms or their components to make useful products. 20. 1 dna sequencing and dna cloning are valuable tools for genetic engineering and biological inquiry. Nucleic acid hybridization- the base pairing of one strand of a nucleic acid to the complementary sequence on a strand from another nucleic acid molecule; key to many techniques used in biological research today. Genetic engineering- the direct manipulation of genes for practical purposes. Dna sequencing: dna sequencing- a process where researchers can exploit the principle of complementary base pairing to determine the complete nucleotide sequence of a dna molecule. Dna is first cut into fragments, and then each fragment is sequenced: sequencing is now carried out by machines, the first automated procedure used a technique called dideoxyribonucleotide chain termination sequencing.
