BIO 362 Lecture Notes - Lecture 23: Prokaryotic Small Ribosomal Subunit, Helicase, Start Codon
Document Summary
Alternative splicing, all of translation on next midterm. Large amino acids cannot enter active site; smaller can get in, but do not fit as well additional screening for second filter. Two classes of trna synthetase enzymes (class i conformational change in binding- cca is in an altered bending conform); class ii is dimeric and binds to trnas of same type to activate it. Each synthetase recognizes unique parts of trna- not all have an editting site. Linked mini helix ((cid:1009)" a(cid:374)d (cid:1007)"(cid:895)- charged by enzyme as long as it has g-u pair- alanine. Ribsome machienry reads aminoacyl-trnas and mrna; 30s + 50s create functional 70s ribosome. Multiple ribosomes engage on the same message mrna (polysomes) Key binding sites (antiobiotics)- functional- mrna binding channel where mrna fits in; 3 sites for trna binding- a to sample codon, p for growing polypeptide associated with trna,
