MBBB 301 Lecture Notes - Lecture 2: Gc-Content, National Center For Biotechnology Information, Intron

• School: Towson University
• Department: Molecular Biology, Biochemistry and Bioinformatics
• Course: MBBB 301
• Professor: All

Document Summary

Gc content: human and most vertebrae have less gc (45-55% max). if see 80%, know its not human codon frequency: can count codon freq. Different species use diffeent codon to code (3 or more code for aa in humans). Amino acid (aa) frequency with nucleotides: can help determine structure of codons (more hydrophobic/hydrophilic aa affect protein struct) Gc content important because know how much need to denature and helps classify organisms (if 80% gc, its not human) Higher gc=higher temp to denature codon frequency- exon identification. High ct=splice site at end of introns. Database- comp software designed to store large amounts of data. Database searching allows searching known sequence similar to query. Searching database- need accession number, name of gene or sequence of gene. Databank- organized archiving info with tools to access info. Kinds of sequences: aa or nucleotide, genomic or mrna/cdna, complete fragmentary sequence (est) Ncbi is part of national library of medicine.