BSC 118 Lecture 20: BSC 118 Chapter 20 Notes
29 Nov 2016
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Document Summary
Gene cloning: to work directly with specific genes, scientists must prepare gene-sized fragments of dna in identical copies, a process called gene cloning, or simply. Applications of recombinant dna technology: recombinant dna technology is widely used in biotechnology, medicine, research, and industry. Research on genome organization, gene expression, and gene function. Large-scale production of proteins used in disease therapy and research. Transgenic organisms genetically modifications organisms, including disease therapy; potentially promising approach but very limited success thus far. Forensics scientific tests and techniques, including pcr, used in connection with the detection and investigation of crimes. Heating, referred to as denaturation in a pcr, breaks the hydrogen bonds between nitrogenous bases of the double-stranded dna sample containing the segment of dna targeted for amplification, thus converting it to single-stranded dna. Cooling, referred to as the annealing step in a pcr, makes possible the binding of short dna polynucleotides (primers) complementary to the ends of the dna sequence targeted for amplification.
