BSC 300 Lecture Notes - Lecture 21: Troponin, Opsonin, Fimbrin

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Important concepts to consider about the dynamics of actin filaments: actin filament polymerization. In vitro mechanism: kinetics, critical concentration, regulation of actin filament polymerization and stability by g-actin and f-actin binding protein. Some gelsolin family members are activated by a rise in ca2+ concentration (-) end capping protein: tropomodulin blocks the end where filament disassembly normally occurs, thereby stabilizing the filament. Inactive state: formin folds back on itself to inhibit fh2 domain activity: activation, membrane receptor activation of rho to the gtp-bound form (rho-gtp) Fusion of the membrane projections pinches off a phagosome into the cytoplasm: step 4: fusion of lysosomes with the phagosome delivers enzymes that degrade the bacterium. Important concepts to remember about myosin-powered movements: myosin ii contraction: Skeletal sarcomere contractile unit-- actin thin filament-- myosin ii thick filament structure stabilized by thin and thick filament associated proteins: atp hydrolysis drives sliding filament sarcomere contraction. Skeletal muscle contraction-- thin filament ca2+ regulation: myosin v regulation and cargo transport.

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