BIO 201 Lecture Notes - Lecture 9: Microtubule Organizing Center, Protein Subunit, Cytoskeleton
20 Feb 2017
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Can be separated based on charge, size, solubility, etc. Sds (detergent, disrupts noncovalent interactions; bind to proteins giving them negative charge) Detergents - amphipathic molecules (vehicle for nonpolar things) # of sds molecules bound to polypeptide is proportional to length of polypeptide. Heat (boiling) - works with sds to disrupt noncovalent interactions. Small proteins migrate faster due to negative charge and small size. Anode (+) at the bottom while cathode (-) at top. Throughout cell, emerging from microtubule organizing center. We know mitotic spindle, mt in cytoplasm of normal cell, and cilia are mts. Alpha and beta tubulin subunits form heterodimers. Polymers only in the presence of gtp. Tubulin (degrades gtp gtpase) catalyzes gtp gdp + p. Polymerization end is (+) while depolymerization end is (-) Dynamic equilibrium of polymerization/depolymerization = dynamic instability/treadmilling. Made from centriole (mt) and other proteins. Mt organizing center in plants and animals. Polymer but has only 1 protein subunit.
