BIO 205 Lecture Notes - Lecture 32: Malate Dehydrogenase, Citric Acid Cycle, Beta Oxidation

1. Which is an w-3 fatty acid?

A) B) C) D) E) None ofthese

2. In many cases, cellular signal transduction is mediated by amembrane-associated G-protein complex. Which is not right aboutthis protein complex?

A) The complex is made of α-, β-, and γ-subunit.

B) The G_sα-subunit has a GTPase activity.

C) Depending on the receptor types, eitherG_sα-subunit or G_iα-subunit is used.

D) Signal transduction through G-proteins ultimately regulatesprotein phosphorylation.

E) None of the above.

3. Which statement is not right about the pyruvatedehydrogenase?

A) This enzyme is located in the mitochondria.

B) This enzyme consists of three different enzymatic subunits(E1, E2, and E3).

C) This enzyme catalyzes the reaction: Pyruvate + CoA + NAD ®Acetyl-CoA + CO₂ + NADH

D) This enzyme also uses TPP (thiamine pyrophosphate), lipoicacid, and FAD as cofactors.

E) Despite its complexity, this enzyme is not an importantregulation point of TCA cycle.

4. To maintain a proper rate of the TCA cycle, oxaloacetate isreplenished periodically. To increase [oxaloacetate], ___X____ isturned on but the rest step of TCA cycled is turned off byphosphorylation of __Y__.

A) X = Glyoxylate cycle :::::: Y = isocitrate dehydrogenase

B) X = Glyoxylate cycle :::::: Y = isocitrate lyase

C) X = Qcycle :::::: Y = isocitrate dehydrogenase

D) X = Qcycle :::::: Y = isocitrate lyase

E) None of the above.

5. Assume you have three NADH and one FADH₂.Calculate the total numbers of H⁺ pumped into theinter-membrane space and the maximal number of ATP produced insidethe matrix.

A) 40 H⁺ and 12 ATP
B) 40 H⁺ and 11 ATP
C) 36 H⁺ and 12 ATP

D) 36 H⁺ and 11 ATP
E) None of the above.

I put the following

1. D

2. D

3. E.

4. idk

5. idk

Can someone help me out for 4 & 5 and reassure I got 1-3correct! Thanks