MIC 301 Lecture Notes - Lecture 4: Biofilm, Protozoa, Hydroxyl Radical
Document Summary
Explain the general principles of microscopy in a few words: Wavelength: various of radiation differ in wavelength, the distance between. 2 parts of wave, 400nm as violet and 650 nm as red, 550 nm as white. Magnification: the apparent increase in size of an object. Number and x, the lens is optically dense. A convex lens focuses light on a focal point, the image is enlarged, inverted and reversed. Light passing through a lens refracts (bends) since light rays slow down as they enter the glass. Resolution: resolving power, the ability to distinguish objects that are close together. The resolution distance is dependent of the wavelength of the radiation and the numerical aperture of the lens (the ability of a lens to gather light) Contrast: differences in intensity between 2 objects or between an object and its background, imp in deterring resolution. Stain and use light in phase (all of the waves" crests and troughs are aligned) can enhance contrast.