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Lecture 1

BSCI 223 Lecture Notes - Lecture 1: Silent Mutation, Glutamine


Department
Biological Sciences Program
Course Code
BSCI 223
Professor
mciver
Lecture
1

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PAK 2.2A
If the nucleotides in the gene were changed as indicated in the
mistake sequence, what type of mutation would this be? Explain.
A silent mutation because the blast alignment from the query and
subject sequence indicates that there is no difference. Site 1557 of the
query sequence is A, and site 1557 of the subject sequence is also A. A
silent mutation shows no change.
[lecture]
Pak 2.2 B
The DNA sequence shown above is the sense (+) strand of the
double stranded DNA that makes up the pmoC gene, expected to
encode PmoC. What would the mRNA sequence be when the
template (-) strand DNA is transcribed? (give first 60 bases) What
would the amino acid sequence be when the mRNA is translated?
Original: GTCGACTGGG CACCAGCCGG ATGCGTCCGT
CAACCCCGAC TGTTCCGCCA AGAACTCCGG
After Transcription: CAGCUGACCC GUCCUCGGCC UACGCAGGCA
GUUGGGGCUG ACAAGGCGGU UCUUGAGGCC
After Translation: Gln Leu Thr Arg Pro Arg Pro Thr Gln Ala Val Gly Ala
Asp Lys Ala Val Leu Glu Ala
[lecture]
PAK 2.1 C
How is the protein product of this DNA sequence different from the
PmoC that would be made if the gene did not have a mutation?
Make a prediction as to the functionality of this altered protein
compared to PmoC.
Silent mutations result in no effect on the protein product. A change on a
nucleotide may still code for the same amino acid as if it were unmutated.
When amino acids stay the same, so does the phenotype of structure and
function.
[lecture]
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