BIOL 2081C Lecture Notes - Lecture 7: Bamhi, Southern Blot, Hindiii
Document Summary
Reading assignment: hartl, sections 12. 1-12. 3 and 12. 5 - 12. 6, focusing on the learning objectives. Learning objectives: identify restriction sites in a particular dna fragment and the cloning site of a vector that would enable the fragment to be inserted into the vector in a prescribed orientation. The target gene gets interrupted and replaced by unrelated base pairs. This can make the dna strand longer which will likely be noticed at the top of a gel electrophoresis: describe and differentiate between the methods used to create transgenic bacteria, mammals, flies, and plants. Method of incorporation w/ host genome: ti plasmid inserts dna into host cell. Method of incorporation w/ host genome: transposase gene allows insertion of everything between p-elements into host genome. Method of incorporation w/ host genome: restriction enzyme, digest/ligation/transformation of plasmid. Plants that break down mercury- bio remediation. Mice with cystic fibrosis so we can test them- use as models of disease.
