BIOL 3077 Lecture Notes - Lecture 4: Allele-Specific Oligonucleotide, Restriction Fragment Length Polymorphism, Point Mutation
Document Summary
Id rare blood types: explain why rflp data were considered more informative than allele-specific pcr data. Interpret an rflp autoradiogram as used in forensics in terms of: match vs exclusion, the source, purpose and use of "dna ladders" A solution of double-stranded dna fragments whose molecule weights (and number of base pairs) are known and standardized. For example, if a forensic sample is a water-based solution, the reference sample must be a water-based solution. In addition, a reference sample contains a precisely defined amount of a target compound or microorganism. Notes: allele specific pcr, use a protein coding gene where we already knew there was a polymorphism (multiple alleles, find place in gene where there was a dna mutation that cause difference in alleles, use.
