BIO 110 Lecture Notes - Lecture 28: Atp Synthase, Carbon Fixation, Light-Dependent Reactions
Document Summary
Much of paper was spent testing other hypotheses other than hypothesis that said new proteins made were new enzymes. Perhaps new proteins made allowed bacteria to survive because bypassed enzyme it was missing. The active site could"ve shaped the new enzyme. Test: if new proteins bypass pathway, then new proteins should rescue mutations at different genes in the same pathway (used citrate synthase) Results: no rescue of other proteins in pathway. Created 4 new enzymes - eliminated alternative hypotheses. Created a strain of e. coli that was lacking 4 different genes (4 genes that were able to replace with all 4 new de novo proteins) A quadruple knockout (missing 4 essential genes) survived. Learn something new about how enzymes work. Create enzymes for reactions that don"t exist yet (ex: detoxifying environmental pollutant, new way to synthesize drugs) Taking pre-existing pathogen and replacing a lot of enzymes more resistant to drugs, antibiotics, or own immune systems.
