BIO 110 Lecture Notes - Lecture 13: Sodium Bicarbonate, Light-Independent Reactions, Cellular Respiration

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Photosynthesis is the metabolic process carried out by plants and some bacteria in which visible light is trapped and the energy is used to convert co2 into organic compounds. Photosynthesis can be divided into a first step: the absorption of photons by chlorophyll raises electrons to higher energy levels. The potential energy in these high-energy electrons is then used to reduce nadp and to generate atp by a process similar to that of oxidative phosphorylation in mitochondria. The second step of photosynthesis is the utilization of atp and nadph by the calvin cycle generates glyceraldehyde 3-phosphate, which can be subsequently converted to sugars. While whole leaves can be placed in a respirometer, because leaf sizes can vary, greater reproducibility can be obtained by using uniform leaf disks", generated with a hollow 2 cylindrical punch. The disks are floated on a solution containing glucose for respiration and sodium bicarbonate (nahco3).

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